摘 要：本研究用一种简便方法提取大鼠脑线粒体DNA(mtDNA)，所得的线粒体DNA纯度为1．8±0．01，得率为每克脑组织0．8～1．0μg线粒体DNA，以此为模板进行PCR反应，扩增线粒体DNA编码基因，产量丰富、特异性高。该方法操作简便、成本低，适用于一般实验室。
Isolation of the Mitochondrial DNA from Rat Brain
xiang Ping,Gao Guoquan,Yao Zhibin(Department of Anatomy, Bengbu Medical College, Bengbu, 233003)
Abstract：A simple technique for isolation of mitochondrial DNA from rat brain is described. The purity (260/280) of mtDNA extracted by the method is 1.8±0. 01, the mtDNA yield per gram brain is 0. 8-10/μg. The product of the PCR is specific. The method can be used in general laboratory because it costs very low and is easy to operate.
Keywords：mtDNA; rat; PCR参考文献：
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